PRODUCT NAME: Methotrexate Nitroso Impurity 1
Date of Mfg: Sample COA	RETEST DATE: Sample COA

Catalogue Number	NIO-491
Item/Batch Number	Sample COA
Quantity	Sample COA
CAS Number	NA
Mol. Formula	C21H21N9O6 : 2(Na)
Molecular Wt	495.5 : 2(23.0)
Synonyms	Disodium (S)-2-(4-(((2,4-diaminopteridin-6-yl)methyl)(methyl)amino)-N-nitrosobenzamido)-2-methylpentanedioate
Long Term Storage Condition	Keep container tightly closed in a dry and well-ventilated place
Handling instruction	Sample COA

Test	Results
Appearance
Water content by KF/TGA	Sample COA
% Potency = [100 - (Water content by KF)] X HPLC Purity)] / 100	Sample COA
Identification by HNMR	Sample COA
Identification By IR	Sample COA
Mass (ESI)	Sample COA
Solubility
Purity	NLT 98%
Analysis Date (Release Date)	Sample COA
Attachments	Sample COA
Other Information	Sample COA
Note: This material can be used as a referance standard and is for research purpose ONLY and not for human consumption