PRODUCT NAME: Amikacin EP Impurity F
Date of Mfg: Sample COA	RETEST DATE: Sample COA

Catalogue Number	AMK-010
Item/Batch Number	Sample COA
Quantity	Sample COA
CAS Number	197909-67-4
Alternate CAS Number	NA(Sulphate Salt)
Mol. Formula	C26H50N6O15
Molecular Wt	686.7 g/mol
IUPAC	(S)-4-Amino-N-((1R,2S,3S,4R,5S)-5-amino-4-(((2R,3R,4S,5S,6R)-6-(((S)-4-amino-2-hydroxybutanamido)methyl)-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl)oxy)
Synonyms	Amikacin Sulfate Impurity F (As per 35.1 EP Monograph); 1,6'-Di-HABA Kanamycin A; (S)-4-Amino-N-((1R,2S,3S,4R,5S)-5-amino-4-(((2R,3R,4S,5S,6R)-6-(((S)-4-amino-2-hydroxybutanamido)methyl)-3,4,5-trihydroxytetrahydro-2H-pyran-2-yl)oxy)-2-(((2S,3R,4S,5S,6R)-4-amino-3,5-dihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)-3-hydroxycyclohexyl)-2-hydroxybutanamide
Long Term Storage Condition	-20°C or lower
Handling instruction	Sample COA

Test	Results
Appearance	white to off-white amorphous powder
Water content by KF/TGA	Sample COA
% Potency = [100 - (Water content by KF)] X HPLC Purity)] / 100	Sample COA
Identification by HNMR	Sample COA
Identification By IR	Sample COA
Mass (ESI)	Sample COA
Solubility
Purity	NLT 98%
Analysis Date (Release Date)	Sample COA
Attachments	Sample COA
Other Information	Sample COA
Note: This material can be used as a referance standard and is for research purpose ONLY and not for human consumption